Direct detection of Mycobacterium tuberculosis in sputum by polymerase chain reaction and DNA hybridization.

A polymerase chain reaction (PCR) assay for the rapid diagnosis of pulmonary tuberculosis was developed by using oligonucleotide primers to amplify a fragment of IS6110, an insertion sequence repeated multiple times in the chromosome of Mycobacterium tuberculosis. Sediment obtained from sputa proces...

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Detalhes bibliográficos
Main Authors: Nolte, F S, Metchock, B, McGowan, J E, Edwards, A, Okwumabua, O, Thurmond, C, Mitchell, P S, Plikaytis, B, Shinnick, T
Formato: Artigo
Idioma:Inglês
Publicado em: 1993
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC265631/
https://ncbi.nlm.nih.gov/pubmed/8349753
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