Direct detection of Mycobacterium tuberculosis in sputum by polymerase chain reaction and DNA hybridization.

A polymerase chain reaction (PCR) assay for the rapid diagnosis of pulmonary tuberculosis was developed by using oligonucleotide primers to amplify a fragment of IS6110, an insertion sequence repeated multiple times in the chromosome of Mycobacterium tuberculosis. Sediment obtained from sputa proces...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Nolte, F S, Metchock, B, McGowan, J E, Edwards, A, Okwumabua, O, Thurmond, C, Mitchell, P S, Plikaytis, B, Shinnick, T
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1993
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265631/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8349753/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.31.7.1777-1782.1993
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