Purification of sputum samples through sucrose improves detection of Mycobacterium tuberculosis by polymerase chain reaction.

A method is described for the routine preparation of sputum samples for detection of Mycobacterium tuberculosis by using polymerase chain reaction amplification. Liquefaction of sputum samples with NaOH and subsequent removal of inhibitors of the polymerase reaction with a 50% sucrose centrifugation...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	J Clin Microbiol
Hauptverfasser:	Victor, T, du Toit, R, van Helden, P D
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1992
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265320/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1624570/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.6.1514-1517.1992
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