Purification of sputum samples through sucrose improves detection of Mycobacterium tuberculosis by polymerase chain reaction.

A method is described for the routine preparation of sputum samples for detection of Mycobacterium tuberculosis by using polymerase chain reaction amplification. Liquefaction of sputum samples with NaOH and subsequent removal of inhibitors of the polymerase reaction with a 50% sucrose centrifugation...

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Dades bibliogràfiques

Publicat a:	J Clin Microbiol
Autors principals:	Victor, T, du Toit, R, van Helden, P D
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology (ASM) 1992
Matèries:	Research Article
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265320/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1624570/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.6.1514-1517.1992
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