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Residue Ionization in LpxC Directly Observed by (67)Zn NMR Spectroscopy

The pH dependence of the solid-state (67)Zn NMR lineshapes has been measured for both wild type (WT) and the H265A mutant of Aquifex aeolicus LpxC, each in the absence of substrate (resting state). The (67)Zn NMR spectrum of WT LpxC at pH 6 (prepared at 0 °C) contains two overlapping quadrupole line...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Lipton, Andrew S., Heck, Robert W., Hernick, Marcy, Fierke, Carol A., Ellis, Paul D.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 2008
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC2652226/
https://ncbi.nlm.nih.gov/pubmed/18761443
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja801776c
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