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Two-state selection of conformation-specific antibodies

We present a general strategy for identification of conformation-specific antibodies using phage display. Different covalent probes were used to trap caspase-1 into 2 alternative conformations, termed the on-form and the off-form. These conformation-trapped forms of the protease were used as antigen...

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Autori principali: Gao, Junjun, Sidhu, Sachdev S., Wells, James A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2009
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2651286/
https://ncbi.nlm.nih.gov/pubmed/19208804
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0812952106
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