Expression, purification, crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase from Bacillus anthracis in the presence of pyruvate

Dihydrodipicolinate synthase (DHDPS) catalyses the first committed step in the lysine-biosynthesis pathway in bacteria, plants and some fungi. In this study, the expression of DHDPS from Bacillus anthracis (Ba-DHDPS) and the purification of the recombinant enzyme in the absence and presence of the s...

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Bibliographic Details
Main Authors: Voss, Jarrod E., Scally, Stephen W., Taylor, Nicole L., Dogovski, Con, Alderton, Malcolm R., Hutton, Craig A., Gerrard, Juliet A., Parker, Michael W., Dobson, Renwick C. J., Perugini, Matthew A.
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2009
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2635873/
https://ncbi.nlm.nih.gov/pubmed/19194017
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109000670
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