A Rainbow of Fluoromodules: A Promiscuous scFv Protein Binds to and Activates a Diverse Set of Fluorogenic Cyanine Dyes

[Image: see text] Combined magnetic and fluorescence cell sorting were used to select Fluorogen Activating Proteins (FAPs) from a yeast surface-displayed library for binding to the fluorogenic cyanine dye Dimethyl Indole Red (DIR). Several FAPs were selected that bind to the dye with low nanomolar K...

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Main Authors: Özhalici-Ünal, Hayriye, Pow, Crystal Lee, Marks, Sarah A., Jesper, Lawrence D., Silva, Gloria L., Shank, Nathaniel I., Jones, Elizabeth W., Burnette, James M., Berget, Peter B., Armitage, Bruce A.
Format: Artigo
Sprog:Inglês
Udgivet: 2008
Fag:
Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2633110/
https://ncbi.nlm.nih.gov/pubmed/18761447
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja805042p
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