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A Rainbow of Fluoromodules: A Promiscuous scFv Protein Binds to and Activates a Diverse Set of Fluorogenic Cyanine Dyes
[Image: see text] Combined magnetic and fluorescence cell sorting were used to select Fluorogen Activating Proteins (FAPs) from a yeast surface-displayed library for binding to the fluorogenic cyanine dye Dimethyl Indole Red (DIR). Several FAPs were selected that bind to the dye with low nanomolar K...
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| Main Authors: | , , , , , , , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2008
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2633110/ https://ncbi.nlm.nih.gov/pubmed/18761447 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja805042p |
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