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Postcrystallization Analysis of the Irreproducibility of the Human Intrinsic Factor—Cobalamin Complex Crystals

Approximately 15% (w/w) of human intrinsic factor (IF) is comprised of carbohydrate side chains, making crystallization problematic. In addition, IF is sensitive to proteolysis. To understand the role of these factors in crystallization, we carried out dynamic light scattering studies and assessed t...

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Detalhes bibliográficos
Main Authors: Sukumar, N., Mathews, F. S., Gordon, M. M., Ealick, S. E., Alpers, D. H.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2631276/
https://ncbi.nlm.nih.gov/pubmed/19884970
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/cg800509f
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