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A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance
BACKGROUND: Quantitative polymerase chain reaction (QPCR) is a widely applied analytical method for the accurate determination of transcript abundance. Primers for QPCR have been designed on a genomic scale but non-specific amplification of non-target genes has frequently been a problem. Although se...
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| Autori principali: | , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
BioMed Central
2008
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2631021/ https://ncbi.nlm.nih.gov/pubmed/19108745 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-9-633 |
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