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Comparative Enzymology in the Alkaline Phosphatase Superfamily to Determine the Catalytic Role of an Active Site Metal Ion

Mechanistic models for biochemical systems are frequently proposed from structural data. Site-directed mutagenesis can be used to test the importance of proposed functional sites, but these data do not necessarily indicate how these sites contribute to function. Herein we apply an alternative approa...

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Detalhes bibliográficos
Main Authors: Zalatan, Jesse G., Fenn, Timothy D., Herschlag, Daniel
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2622731/
https://ncbi.nlm.nih.gov/pubmed/18851975
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2008.09.059
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