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Cloning and expression of the multifunctional human fatty acid synthase and its subdomains in Escherichia coli

We engineered a full-length (8.3-kbp) cDNA coding for fatty acid synthase (FAS; EC 2.3.1.85) from the human brain FAS cDNA clones we characterized previously. In the process of accomplishing this task, we developed a novel PCR procedure, recombinant PCR, which is very useful in joining two overlappi...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Jayakumar, Arumugam, Huang, Wei-Yong, Raetz, Beate, Chirala, Subrahmanyam S., Wakil, Salih J.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1996
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC26163/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8962082/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.25.14509
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