Cloning and expression of the multifunctional human fatty acid synthase and its subdomains in Escherichia coli
We engineered a full-length (8.3-kbp) cDNA coding for fatty acid synthase (FAS; EC 2.3.1.85) from the human brain FAS cDNA clones we characterized previously. In the process of accomplishing this task, we developed a novel PCR procedure, recombinant PCR, which is very useful in joining two overlappi...
में बचाया:
| में प्रकाशित: | Proc Natl Acad Sci U S A |
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| मुख्य लेखकों: | , , , , |
| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
National Academy of Sciences
1996
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC26163/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8962082/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.25.14509 |
| टैग: |
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