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Efficient isolation of soluble intracellular single-chain antibodies using the twin-arginine translocation machinery

One of the most commonly used recombinant antibody formats is the single-chain variable fragment (scFv) that consists of the antibody variable heavy chain connected to the variable light chain by a flexible linker. Since disulfide bonds are often necessary for scFv folding, it can be challenging to...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Fisher, Adam, DeLisa, Matthew P.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2008
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC2612092/
https://ncbi.nlm.nih.gov/pubmed/18992254
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2008.10.051
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