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Efficient isolation of soluble intracellular single-chain antibodies using the twin-arginine translocation machinery
One of the most commonly used recombinant antibody formats is the single-chain variable fragment (scFv) that consists of the antibody variable heavy chain connected to the variable light chain by a flexible linker. Since disulfide bonds are often necessary for scFv folding, it can be challenging to...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2612092/ https://ncbi.nlm.nih.gov/pubmed/18992254 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2008.10.051 |
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