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Twin-Arginine Translocation of Active Human Tissue Plasminogen Activator in Escherichia coli

When eukaryotic proteins with multiple disulfide bonds are expressed at high levels in Escherichia coli, the efficiency of thiol oxidation and isomerization is typically not sufficient to yield soluble products with native structures. Even when such proteins are secreted into the oxidizing periplasm...

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Bibliografski detalji
Glavni autori: Kim, Jae-Young, Fogarty, Elizabeth A., Lu, Franklin J., Zhu, Hui, Wheelock, Geoffrey D., Henderson, Lee A., DeLisa, Matthew P.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2005
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1317466/
https://ncbi.nlm.nih.gov/pubmed/16332834
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.12.8451-8459.2005
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