Twin-Arginine Translocation of Active Human Tissue Plasminogen Activator in Escherichia coli

When eukaryotic proteins with multiple disulfide bonds are expressed at high levels in Escherichia coli, the efficiency of thiol oxidation and isomerization is typically not sufficient to yield soluble products with native structures. Even when such proteins are secreted into the oxidizing periplasm...

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Detalhes bibliográficos
Principais autores: Kim, Jae-Young, Fogarty, Elizabeth A., Lu, Franklin J., Zhu, Hui, Wheelock, Geoffrey D., Henderson, Lee A., DeLisa, Matthew P.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Physiology and Biotechnology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1317466/
https://ncbi.nlm.nih.gov/pubmed/16332834
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.12.8451-8459.2005
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