Purification of Clostridium difficile toxin A by affinity chromatography on immobilized thyroglobulin.
An efficient, single-step method for isolating highly purified toxin A from Clostridium difficile culture filtrates is described. The purification procedure was based on the affinity binding and release of toxin A to bovine thyroglobulin conjugated to agarose beads. The toxin strongly bound at 4 deg...
Gardado en:
| Publicado en: | Infect Immun |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1987
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC260617/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3112015/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.55.8.1873-1877.1987 |
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