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Purification of Clostridium difficile toxin A by affinity chromatography on immobilized thyroglobulin.
An efficient, single-step method for isolating highly purified toxin A from Clostridium difficile culture filtrates is described. The purification procedure was based on the affinity binding and release of toxin A to bovine thyroglobulin conjugated to agarose beads. The toxin strongly bound at 4 deg...
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| 主要な著者: | , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
1987
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| 主題: | |
| オンライン・アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC260617/ https://ncbi.nlm.nih.gov/pubmed/3112015 |
| タグ: |
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