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Purification of Clostridium difficile toxin A by affinity chromatography on immobilized thyroglobulin.
An efficient, single-step method for isolating highly purified toxin A from Clostridium difficile culture filtrates is described. The purification procedure was based on the affinity binding and release of toxin A to bovine thyroglobulin conjugated to agarose beads. The toxin strongly bound at 4 deg...
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| Asıl Yazarlar: | , |
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| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
1987
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC260617/ https://ncbi.nlm.nih.gov/pubmed/3112015 |
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