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Combinatorial Incorporation of Enhancer Blocking Components of the Chicken β-Globin 5′HS4 and Human T-Cell Receptor α/δ BEAD-1 Insulators in Self-Inactivating Retroviral Vectors Reduces their Genotoxic Potential

Insertional mutagenesis by retroviral vectors has emerged as a serious impediment to the widespread application of hematopoietic stem cell gene transfer for the treatment of hematologic diseases. Here we report the development of a 77-bp element, FII/BEAD-A (FB), which contains the minimal enhancer...

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Autori principali: RAMEZANI, ALI, HAWLEY, TERESA S., HAWLEY, ROBERT G.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2008
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2605779/
https://ncbi.nlm.nih.gov/pubmed/18787211
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1634/stemcells.2008-0258
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