Introduction of cytochrome b mutations in Saccharomyces cerevisiae by a method that allows selection for both functional and nonfunctional cytochrome b proteins

We have previously used inhibitors interacting with the Qn site of the yeast cytochrome bc(1) complex to obtain yeast strains with resistance-conferring mutations in cytochrome b as a means to investigate the effects of amino acid substitutions on Qn site enzymatic activity (1). Although the screeni...

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Bibliographic Details
Main Authors: Ding, Martina G., Butler, Christine A., Saracco, Scott A., Fox, Thomas D., Godard, François, di Rago, Jean-Paul, Trumpower, Bernard L.
Format: Artigo
Language:Inglês
Published: 2008
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2601669/
https://ncbi.nlm.nih.gov/pubmed/18498758
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbabio.2008.04.029
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