Cloning and expression in Escherichia coli of the Streptococcus pneumoniae gene encoding pneumolysin.

A gene bank of Sau3A1-generated Streptococcus pneumoniae DNA fragments was constructed in Escherichia coli K-12 by cloning into the BamHI site of the cosmid vector pHC79. Clones expressing the pneumolysin determinant were selected by testing for hemolytic activity which could be inhibited by antibod...

Popoln opis

Bibliografske podrobnosti

izdano v:	Infect Immun
Principais autores:	Paton, J C, Berry, A M, Lock, R A, Hansman, D, Manning, P A
Format:	Artigo
Jezik:	Inglês
Izdano:	American Society for Microbiology (ASM) 1986
Teme:	Research Article
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC260115/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3019892/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.54.1.50-55.1986
Oznake:	Označite Brez oznak, prvi označite!