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Cloning and expression in Escherichia coli of the Streptococcus pneumoniae gene encoding pneumolysin.

A gene bank of Sau3A1-generated Streptococcus pneumoniae DNA fragments was constructed in Escherichia coli K-12 by cloning into the BamHI site of the cosmid vector pHC79. Clones expressing the pneumolysin determinant were selected by testing for hemolytic activity which could be inhibited by antibod...

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Udgivet i:Infect Immun
Principais autores: Paton, J C, Berry, A M, Lock, R A, Hansman, D, Manning, P A
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1986
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC260115/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3019892/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.54.1.50-55.1986
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