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Cloning and expression in Escherichia coli of the Streptococcus pneumoniae gene encoding pneumolysin.

A gene bank of Sau3A1-generated Streptococcus pneumoniae DNA fragments was constructed in Escherichia coli K-12 by cloning into the BamHI site of the cosmid vector pHC79. Clones expressing the pneumolysin determinant were selected by testing for hemolytic activity which could be inhibited by antibod...

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Main Authors: Paton, J C, Berry, A M, Lock, R A, Hansman, D, Manning, P A
Format: Artigo
Jezik:Inglês
Izdano: 1986
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC260115/
https://ncbi.nlm.nih.gov/pubmed/3019892
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