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Purification, crystallization and preliminary X-ray diffraction analysis of an oomycete-derived Nep1-like protein
The elicitor protein Nep1-like protein from the plant pathogen Pythium aphanidermatum was purified and crystallized using the hanging-drop vapour-diffusion method. A native data set was collected to 1.35 Å resolution at 100 K using synchrotron radiation. Since selenomethionine-labelled protein did n...
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Main Authors: | , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
International Union of Crystallography
2008
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2593687/ https://ncbi.nlm.nih.gov/pubmed/19052381 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108037640 |
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