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Purification, crystallization and preliminary X-ray diffraction analysis of an oomycete-derived Nep1-­like protein

The elicitor protein Nep1-like protein from the plant pathogen Pythium aphanidermatum was purified and crystallized using the hanging-drop vapour-diffusion method. A native data set was collected to 1.35 Å resolution at 100 K using synchrotron radiation. Since selenomethionine-labelled protein did n...

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Detalhes bibliográficos
Main Authors: Luberacki, Borries, Weyand, Michael, Seitz, Ulrich, Koch, Wolfgang, Oecking, Claudia, Ottmann, Christian
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2008
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2593687/
https://ncbi.nlm.nih.gov/pubmed/19052381
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108037640
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