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Cloning, expression, purification and crystallization as well as X-ray fluorescence and preliminary X-ray diffraction analyses of human ADP-ribosylhydrolase 1

Human ADP-ribosylhydrolase 1 (hARH1, ADPRH) cleaves the glycosidic bond of ADP-ribose attached to an Arg residue of a protein. hARH1 has been cloned, expressed heterologously in Escherichia coli, purified and crystallized in complex with K(+) and ADP. The orthorhombic crystals contained one monomer...

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Detalhes bibliográficos
Main Authors: Kernstock, Stefan, Koch-Nolte, Friedrich, Mueller-Dieckmann, Jochen, Weiss, Manfred S., Mueller-Dieckmann, Christoph
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2675603/
https://ncbi.nlm.nih.gov/pubmed/19407395
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109014067
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