Rapid and reliable universal cloning of influenza A virus genes by target-primed plasmid amplification

Reverse genetics has become pivotal in influenza virus research relying on rapid generation of tailored recombinant influenza viruses. They are rescued from transfected plasmids encoding the eight influenza virus gene segments, which have been cloned using restriction endonucleases and DNA ligation....

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Библиографические подробности
Главные авторы: Stech, Jürgen, Stech, Olga, Herwig, Astrid, Altmeppen, Hermann, Hundt, Jana, Gohrbandt, Sandra, Kreibich, Anne, Weber, Siegfried, Klenk, Hans-Dieter, Mettenleiter, Thomas C.
Формат: Artigo
Язык:Inglês
Опубликовано: Oxford University Press 2008
Предметы:
Methods Online
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2588516/
https://ncbi.nlm.nih.gov/pubmed/18832366
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn646
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