Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification

We describe a simple method of using rolling circle amplification to amplify vector DNA such as M13 or plasmid DNA from single colonies or plaques. Using random primers and φ29 DNA polymerase, circular DNA templates can be amplified 10,000-fold in a few hours. This procedure removes the need for len...

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Detalles Bibliográficos
Main Authors: Dean, Frank B., Nelson, John R., Giesler, Theresa L., Lasken, Roger S.
Formato: Artigo
Idioma:Inglês
Publicado: Cold Spring Harbor Laboratory Press 2001
Assuntos:
Methods
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC311129/
https://ncbi.nlm.nih.gov/pubmed/11381035
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.180501
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