Rapid and reliable universal cloning of influenza A virus genes by target-primed plasmid amplification

Reverse genetics has become pivotal in influenza virus research relying on rapid generation of tailored recombinant influenza viruses. They are rescued from transfected plasmids encoding the eight influenza virus gene segments, which have been cloned using restriction endonucleases and DNA ligation....

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Detalhes bibliográficos
Main Authors: Stech, Jürgen, Stech, Olga, Herwig, Astrid, Altmeppen, Hermann, Hundt, Jana, Gohrbandt, Sandra, Kreibich, Anne, Weber, Siegfried, Klenk, Hans-Dieter, Mettenleiter, Thomas C.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2008
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2588516/
https://ncbi.nlm.nih.gov/pubmed/18832366
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn646
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