Expression of recombinant Clostridium difficile toxin A and B in Bacillus megaterium

BACKGROUND: Major Clostridium difficile virulence factors are the exotoxins TcdA and TcdB. Due to the large size and poor stability of the proteins, the active recombinant TcdA and TcdB have been difficult to produce. RESULTS: The toxin genes tcdA and tcdB were amplified by PCR using chromosomal DNA...

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Библиографические подробности
Главные авторы: Yang, Guilin, Zhou, Boping, Wang, Jufang, He, Xiangyun, Sun, Xingmin, Nie, Weijia, Tzipori, Saul, Feng, Hanping
Формат: Artigo
Язык:Inglês
Опубликовано: BioMed Central 2008
Предметы:
Methodology Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2586027/
https://ncbi.nlm.nih.gov/pubmed/18990232
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2180-8-192
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