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Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome

The construction of cDNA clones encoding large-size RNA molecules of biological interest, like coronavirus genomes, which are among the largest mature RNA molecules known to biology, has been hampered by the instability of those cDNAs in bacteria. Herein, we show that the application of two strategi...

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Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Almazán, Fernando, González, José M., Pénzes, Zoltan, Izeta, Ander, Calvo, Enrique, Plana-Durán, Juan, Enjuanes, Luis
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: The National Academy of Sciences 2000
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC25860/
https://ncbi.nlm.nih.gov/pubmed/10805807
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