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Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes

The construction of coronavirus (CoV) infectious clones had been hampered by the large size of the viral genome (around 30 kb) and the instability of plasmids carrying CoV replicase sequences in Escherichia coli. Several approaches have been developed to overcome these problems. Here we describe the...

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Détails bibliographiques
Publié dans:SARS- and Other Coronaviruses
Auteurs principaux: Almazán, Fernando, Galán, Carmen, Enjuanes, Luis
Format: Artigo
Langue:Inglês
Publié: 2007
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7121107/
https://ncbi.nlm.nih.gov/pubmed/19057870
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-59745-181-9_20
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