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Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
The construction of coronavirus (CoV) infectious clones had been hampered by the large size of the viral genome (around 30 kb) and the instability of plasmids carrying CoV replicase sequences in Escherichia coli. Several approaches have been developed to overcome these problems. Here we describe the...
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| Опубликовано в: : | SARS- and Other Coronaviruses |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2007
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7121107/ https://ncbi.nlm.nih.gov/pubmed/19057870 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-59745-181-9_20 |
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