Stabilization of a Full-Length Infectious cDNA Clone of Transmissible Gastroenteritis Coronavirus by Insertion of an Intron

The stable propagation of a full-length transmissible gastroenteritis coronavirus (TGEV) cDNA in Escherichia coli cells as a bacterial artificial chromosome has been considerably improved by the insertion of an intron to disrupt a toxic region identified in the viral genome. The viral RNA was expres...

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Detalles Bibliográficos
Main Authors: González, José M., Pénzes, Zoltan, Almazán, Fernando, Calvo, Enrique, Enjuanes, Luis
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2002
Assuntos:
Replication
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC155106/
https://ncbi.nlm.nih.gov/pubmed/11932433
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.76.9.4655-4661.2002
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