Purification, crystallization and X-ray diffraction analysis of pavine N-methyltransferase from Thalictrum flavum

A cDNA from the plant Thalictrum flavum encoding pavine N-methyltrans­ferase, an enzyme belonging to a novel class of S-adenosylmethionine-dependent N-methyltransferases specific for benzylisoquinoline alkaloids, has been heterologously expressed in Escherichia coli. The enzyme was purified using af...

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Detalhes bibliográficos
Main Authors: Jain, Ankur, Ziegler, Jörg, Liscombe, David K., Facchini, Peter J., Tucker, Paul A., Panjikar, Santosh
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2008
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2581683/
https://ncbi.nlm.nih.gov/pubmed/18997344
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108033046
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