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Cloning, expression, crystallization and preliminary X-ray data analysis of norcoclaurine synthase from Thalictrum flavum

Norcoclaurine synthase (NCS) catalyzes the condensation of 3,4-dihydroxy­phenylethylamine (dopamine) and 4-hydroxyphenylacetaldehyde (4-HPAA) as the first committed step in the biosynthesis of benzylisoquinoline alkaloids in plants. The protein was cloned, expressed and purified. Crystals were obtai...

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Detalhes bibliográficos
Main Authors: Pasquo, Alessandra, Bonamore, Alessandra, Franceschini, Stefano, Macone, Alberto, Boffi, Alberto, Ilari, Andrea
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2374240/
https://ncbi.nlm.nih.gov/pubmed/18391427
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108005678
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