Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of SAICAR synthase from Streptococcus suis serotype 2

Phosphoribosylaminoimidazole-succinocarboxamide synthase (SAICAR syn­thase) plays an essential role in the de novo biosynthesis of purine nucleotides. In this study, the SAICAR synthase from Streptococcus suis was cloned and overexpressed in Escherichia coli. The subsequent product was purified and...

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Detalhes bibliográficos
Main Authors: Cheng, Xia, Lu, Guangwen, Qi, Jianxun, Cheng, Hao, Gao, Feng, Wang, Jundong, Yan, Jinghua
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2010
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2917288/
https://ncbi.nlm.nih.gov/pubmed/20693665
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110020518
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