Construction and expression of the complete Clostridium difficile toxin A gene in Escherichia coli.

Cloned fragments constituting the 8.1-kb toxin A gene of Clostridium difficile were used to reconstruct the intact gene. The recombinant toxin expressed in Escherichia coli was cytotoxic, enterotoxic, and lethal. In addition, toxic lysate caused hemagglutination of rabbit erythrocytes. The toxic act...

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Detalhes bibliográficos

Publicado no:	Infect Immun
Principais autores:	Phelps, C J, Lyerly, D L, Johnson, J L, Wilkins, T D
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC257719/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1987027/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.59.1.150-153.1991
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