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Construction and expression of the complete Clostridium difficile toxin A gene in Escherichia coli.

Cloned fragments constituting the 8.1-kb toxin A gene of Clostridium difficile were used to reconstruct the intact gene. The recombinant toxin expressed in Escherichia coli was cytotoxic, enterotoxic, and lethal. In addition, toxic lysate caused hemagglutination of rabbit erythrocytes. The toxic act...

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Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Infect Immun
Egile Nagusiak: Phelps, C J, Lyerly, D L, Johnson, J L, Wilkins, T D
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: American Society for Microbiology (ASM) 1991
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC257719/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1987027/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.59.1.150-153.1991
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