Codon Optimization Increases Steady-State mRNA Levels in Aspergillus oryzae Heterologous Gene Expression

We investigated the effect of codon optimization on the expression levels of heterologous proteins in Aspergillus oryzae, using the mite allergen Der f 7 as a model protein. A codon-optimized Der f 7 gene was synthesized according to the frequency of codon usage in A. oryzae by recursive PCR. Both n...

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Bibliographic Details
Main Authors: Tokuoka, Masafumi, Tanaka, Mizuki, Ono, Kazuhisa, Takagi, Shinobu, Shintani, Takahiro, Gomi, Katsuya
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2008
Subjects:
Mycology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2576710/
https://ncbi.nlm.nih.gov/pubmed/18791013
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01354-08
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