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Codon Optimization Increases Steady-State mRNA Levels in Aspergillus oryzae Heterologous Gene Expression
We investigated the effect of codon optimization on the expression levels of heterologous proteins in Aspergillus oryzae, using the mite allergen Der f 7 as a model protein. A codon-optimized Der f 7 gene was synthesized according to the frequency of codon usage in A. oryzae by recursive PCR. Both n...
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| Главные авторы: | , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Microbiology (ASM)
2008
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2576710/ https://ncbi.nlm.nih.gov/pubmed/18791013 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01354-08 |
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