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DNA polymerase catalysis in the absence of Watson-Crick hydrogen bonds: Analysis by single-turnover kinetics
We report the first pre-steady-state kinetic studies of DNA replication in the absence of hydrogen bonds. We have used nonpolar nucleotide analogues that mimic the shape of a Watson-Crick base pair in order to investigate the kinetic consequences of a lack of hydrogen bonds in the polymerase reactio...
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| Main Authors: | , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2006
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2567902/ https://ncbi.nlm.nih.gov/pubmed/16411765 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi051792i |
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