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Maintenance of undifferentiated mouse embryonic stem cells in suspension by the serum- and feeder-free defined culture condition

The proven pluripotency of ES cells is expected to allow their therapeutic use for regenerative medicine. We present here a novel suspension culture method that facilitates the proliferation of pluripotent ES cells without feeder cells. The culture medium contains polyvinyl alcohol (PVA), free of ei...

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Hlavní autoři: Tsuji, Yukiiko, Yoshimura, Naoko, Aoki, Hitomi, Sharov, Alexei A., Ko, Minoru S.H., Motohashi, Tsutomu, Kunisada, Takahiro
Médium: Artigo
Jazyk:Inglês
Vydáno: 2008
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2559871/
https://ncbi.nlm.nih.gov/pubmed/18624284
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/dvdy.21617
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