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Maintenance of undifferentiated mouse embryonic stem cells in suspension by the serum- and feeder-free defined culture condition
The proven pluripotency of ES cells is expected to allow their therapeutic use for regenerative medicine. We present here a novel suspension culture method that facilitates the proliferation of pluripotent ES cells without feeder cells. The culture medium contains polyvinyl alcohol (PVA), free of ei...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2559871/ https://ncbi.nlm.nih.gov/pubmed/18624284 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/dvdy.21617 |
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