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Analysis of the Factors that Limit the Ability of Feeder Cells to Maintain the Undifferentiated State of Human Embryonic Stem Cells

Human embryonic stem cell (hESC) culture is routinely performed using inactivated mouse embryonic fibroblasts (MEFs) as a feeder cell layer (FL). Although these cells maintain pluripotency of hESCs, the molecular basis for this is unknown. Objectives of this study were to determine whether timing be...

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Autori principali: Villa-Diaz, Luis G., Pacut, Crystal, Slawny, Nicole A., Ding, Jun, O'Shea, K. Sue, Smith, Gary D.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Mary Ann Liebert, Inc. 2009
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3133563/
https://ncbi.nlm.nih.gov/pubmed/18764735
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/scd.2008.0010
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