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Following translation by single ribosomes one codon at a time

We have followed individual ribosomes as they translate single messenger RNA hairpins tethered by the ends to optical tweezers. Here we reveal that translation occurs through successive translocation-and-pause cycles. The distribution of pause lengths, with a median of 2.8 s, indicates that at least...

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Detalhes bibliográficos
Main Authors: Wen, Jin-Der, Lancaster, Laura, Hodges, Courtney, Zeri, Ana-Carolina, Yoshimura, Shige H., Noller, Harry F., Bustamante, Carlos, Tinoco, Ignacio
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2556548/
https://ncbi.nlm.nih.gov/pubmed/18327250
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nature06716
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