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Pentalenolactone Biosynthesis. Molecular Cloning and Assignment of Biochemical Function to PtlH, a Non-Heme Iron Dioxygenase of Streptomyces avermitilis
[Image: see text] The hydroxylase encoded by theptlH (SAV2991) gene from the pentalenolactone gene cluster ofStreptomyces avermitilis was cloned by PCR and expressed inEscherichia colias an N-terminal-His(6)-tag protein. Incubation of recombinant PtlH with (±)-1- deoxypentalenic acid (5) in the pres...
Bewaard in:
| Hoofdauteurs: | , , , |
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| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
2006
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2533727/ https://ncbi.nlm.nih.gov/pubmed/16704250 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja061469i |
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