Dehydration of main-chain amides in the final folding step of single-chain monellin revealed by time-resolved infrared spectroscopy

Kinetic IR spectroscopy was used to reveal β-sheet formation and water expulsion in the folding of single-chain monellin (SMN) composed of a five-stranded β-sheet and an α-helix. The time-resolved IR spectra between 100 μs and 10 s were analyzed based on two consecutive intermediates, I(1) and I(2),...

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Main Authors: Kimura, Tetsunari, Maeda, Akio, Nishiguchi, Shingo, Ishimori, Koichiro, Morishima, Isao, Konno, Takashi, Goto, Yuji, Takahashi, Satoshi
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2008
Teme:
Biological Sciences
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2533200/
https://ncbi.nlm.nih.gov/pubmed/18757727
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0801316105
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