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Uncovering dehydration in cytochrome c refolding from urea- and guanidine hydrochloride-denatured unfolded state by high pressure spectroscopy

To investigate the dehydration associated with protein folding, the partial molar volume changes for protein unfolding (ΔV(u)) in cytochrome c (Cyt c) were determined using high pressure absorption spectroscopy. ΔV(u) values for the unfolding to urea- and guanidine hydrochloride (GdnHCl)-denatured C...

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Detalhes bibliográficos
Publicado no:Biophys Physicobiol
Main Authors: Konno, Shohei, Doi, Kentaro, Ishimori, Koichiro
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society of Japan (BSJ) 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6373425/
https://ncbi.nlm.nih.gov/pubmed/30775200
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2142/biophysico.16.0_18
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